Application

Immunohistochemistry: Use at 5 - 10 µg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.

Western blot: 1 - 10 µg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.

ELISA: Use at 1.0 µg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH.

Research Sub CategoryOxidative Stress

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA.

Specificity

Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing.